Activation of immunoglobulin kappa gene rearrangement correlates with induction of germline kappa gene transcription
Identifieur interne : 004B50 ( Main/Exploration ); précédent : 004B49; suivant : 004B51Activation of immunoglobulin kappa gene rearrangement correlates with induction of germline kappa gene transcription
Auteurs : Mark S. Schlissel [États-Unis] ; David Baltimore [États-Unis]Source :
- Cell [ 0092-8674 ] ; 1989.
English descriptors
- Teeft :
- Activates, Activates transcription, Amplification, Assay, Boehringer mannheim, Cell line, Cell lines, Cell population, Cell populations, Constant amount, Cultured cells, Degenerate, Experimental procedures, Gene, Gene rearrangement, Gene segments, Genome, Germline, Germline configuration, Heavy chain alleles, Heavy chain gene, Immunoglobulin, Immunoglobulin kappa gene rearrangement, Kappa, Kappa gene rearrangement, Kappa gene rearrangements, Kappa genes, Kappa loci, Light chain genes, Locus, Lysis buffer, Polymerase, Polymerase chain reaction, Primer, Primer population, Protein synthesis, Reaction products, Rearrangement, Rearrangement frequency, Region gene, Room temperature, Sensitive assay, Southern blot, Transcription, Transcription factor, Transcriptional, Transcriptional regulation, Unrearranged, Unrearranged gene transcription, Unrearranged kappa, Unrearranged kappa genes, Untreated cultures, Wehi, Yancopoulos.
Abstract
Abstract: We have developed a sensitive polymerase chain reaction assay for measuring the fraction of rearranged immunoglobulin kappa genes in a cell population. Using this assay with Abelson virus-transformed murine pre-B cells, we have found that bacterial lipopolysacharide treatment, which activates transcription of the unrearranged kappa constant region gene, also activates kappa gene rearrangement. In addition, we have been able to detect kappa gene rearrangement in cell lines that do not produce a functional heavy chain gene product (mu protein). These results implicate transcription or transcription factor binding as a regulator of immunoglobulin gene rearrangement.
Url:
DOI: 10.1016/0092-8674(89)90951-3
Affiliations:
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Using this assay with Abelson virus-transformed murine pre-B cells, we have found that bacterial lipopolysacharide treatment, which activates transcription of the unrearranged kappa constant region gene, also activates kappa gene rearrangement. In addition, we have been able to detect kappa gene rearrangement in cell lines that do not produce a functional heavy chain gene product (mu protein). These results implicate transcription or transcription factor binding as a regulator of immunoglobulin gene rearrangement.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Massachusetts</li></region></list><tree><country name="États-Unis"><region name="Massachusetts"><name sortKey="Schlissel, Mark S" sort="Schlissel, Mark S" uniqKey="Schlissel M" first="Mark S." last="Schlissel">Mark S. Schlissel</name></region><name sortKey="Baltimore, David" sort="Baltimore, David" uniqKey="Baltimore D" first="David" last="Baltimore">David Baltimore</name><name sortKey="Baltimore, David" sort="Baltimore, David" uniqKey="Baltimore D" first="David" last="Baltimore">David Baltimore</name><name sortKey="Schlissel, Mark S" sort="Schlissel, Mark S" uniqKey="Schlissel M" first="Mark S." last="Schlissel">Mark S. Schlissel</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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